I just started googling the different strains, Sky. Just googled the bisetti and I'm not going further right now, and I'll tell you why. After reading about how the mice were protected from getting Lyme from the tick saliva and realizing how the world of parasites and adaptation works (after reading (again, sorry) Parasite Rex by Carl Zimmer)) I came to realize that those different strains are (PROBABLY, POSSIBLY) Natural adaptations to their own particular environment. I could be wrong. It's happened once or twice in my life , and I could be way off base. But I have ZERO faith in what the NIH or any other govt scientific reports have to say.
"...When the going gets tough, the weird turn pro..." Raoul Duke..Fear and Loathing in Las Vegas "
So, basically, what they did was create something so they can make a vaccine for it and make trillions of dollars. Meanwhile, it's a total farce since it won't work. They are promising false hope and dancing at the bank. People all over the world are suffering from this and it is such a freaking JOKE. There is NO vaccine that will work. Morgellons will still go on.
BUT in actuality it will NOT! They didn't count on the people here figuring out what is happening.
The new disease is characterized by high fever, which relapses without treatment and may be confused with Lyme disease. There are currently no diagnostic tests available, but Yale researchers have recently received a grant from the National Institutes of Health (NIH) to develop a diagnostic test procedure to look for cases of this new disease in the United States.
"This is the first time we will have a chance to identify a new tick-borne disease in the United States based upon evidence that the agent occurs in ticks," said Fish, co-author of the paper and co-investigator on the NIH grant along with Peter Krause, a senior research scientist in the Division of Epidemiology of Microbial Diseases.
Awwwww..... I have such a warm and freaking fuzzy feeling about these NIH guys.
NO tests available, BUT they got a grant so they can figger one out.
Remember Venter was at NIH, in Maryland, then DOE/CELERA, GENOMES 2LIFE with EPA...... Created microbes to clean up the environment, created in the lab. then Venter Institute........
First they played in the environment, experimenting and releasing all kinds of microbes and organisms, then began on humans.
Not sure when they decided to change the entire genome as they sequenced it, they tweaked it...........?
====================
Will try to find a way to get the maps here..............
The one I found covers the most current line around world, seems to be following shorelines. the b. miyo.......and others, recombined just like influenza........Can see the plan can't we. Update it, constantly, recombine it, give it a different vector. so on.....
b. turicatae..................seems to be the same as b. miyomotoi.........
I have it posted on a document. If you can copy or save them to your picture folders, make a folder for them then transfer them to a document Word Document? if you have that.
Then can tell you from there how to do. Unless you want photoshop or simpleload. That is what Bananny used.
Then you can post on here, I believe.
Lymes, HIV, Morgellons and Alzheimers, connected, they found lymes in Alzheimers.
So, it was a progressive recombination of organic, then inorganic/organic and inorganic.
On the way to the machine, deleting those messages from our DNA.
===========================================
Here shows how this was showing up just like Morgellons did Ca, Tx, Fl. and it was called relapsing fever then..................
Phylogenetic Analysis of the Spirochetes Borrelia parkeri and Borrelia turicatae and the Potential for Tick-Borne Relapsing Fever in Florida TABLE 1.
Designations of the B. turicatae and B. parkeri isolates used in this study and their biological sources, years of isolation, and geographic origins
Species and isolate Host source (no. and stage)a Yr Localityb B. turicatae RML O. turicata (?) ? Kansas 91E135 O. turicata (3N, 3A) 1991 Crockett Co., Tex. 95PE-570 O. turicata (1N, 1A) 1995 Atascosa Co., Tex. 99PE-1807 O. turicata (2A) 1999 Real Co., Tex. PE1-926 O. turicata (1A) 1999 Real Co., Tex. TCB-1 Domestic dog 1999 Clay Co., Tex. TCB-2 Domestic dog 2001 Lubbock Co., Tex. FCB Domestic dog 1992 Sumter Co., Fla. B. parkeri RML O. parkeri (?) ? California CA216 O. parkeri (1M) 1991 Monterey Co., Calif. CA218 O. parkeri (1N) 1991 Monterey Co., Calif. CA219 O. parkeri (1F) 1991 Monterey Co., Calif. CA220 O. parkeri (1M) 1991 Monterey Co., Calif. CA221 O. parkeri (1N) 1991 Monterey Co., Calif.
↵a A, adult, sex unknown; M, male; F, female; N, nymph.
↵b Co., County.
SDS-polyacrylamide gel electrophoresis and immunoblot analysis.Whole-cell lysates of spirochetes were prepared as described previously (57). Proteins were separated by one-dimensional sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with Laemmli buffer (45) and a vertical gel apparatus (Bethesda Research Laboratories-GIBCO, Gaithersburg, Md.). Proteins were blotted onto nitrocellulose membranes with Towbin buffer (70) and a Trans-Blot Cell (Bio-Rad Laboratories). The membranes were blocked overnight at room temperature with TSE-Tween (50 mM Tris, pH 7.4, 150 mM NaCl, 5 mM EDTA, 0.05% Tween 20) and incubated with monoclonal antibodies H9724 (8) and H9826 (55). Bound antibodies were detected by 125I-labeled protein A autoradiography.
Oligonucleotide primers used for gene amplification and DNA sequencing
Along comes the Oligonucleotides,,,,,,,,,,artificial/synthetic.
Tie in to Fort Detrick, or NIH ........Maryland? SDS?
CA, TX, FL.................................... 1991-2
Monterey.......CA........1991...............Fort ORD was still going? There is an anthrax story about Fort ORD, they had to shut it down? or something? Made it into a STATE COLLEGE run by Panetta...............................And they also before that had shut down University of California at IRVINE, and started up again in the 90s.............
means IRVINE had something to do with this. Right away began working on genome............
from your link Kritters::::::::::: just what we want TICK SALIVA>.........
On the Trail of a Vaccine for Lyme Disease: Yale Researchers Target Tick Saliva New Haven, Conn. — A protein found in the saliva of ticks helps protect mice from developing Lyme disease, Yale researchers have discovered. The findings, published in the November 19 issue of Cell Host & Microbe, may spur development of a new vaccine against infection from Lyme disease,...
oh my gosh............the IRON and MANGANESE Connection..............
=========
Scientists identify potential key to Lyme disease
DALLAS — Feb. 9, 2009 — Researchers at UT Southwestern Medical Center have identified a protein that may help give Lyme disease its bite.
The findings suggest that the bacterial protein, which aids in transporting the metal manganese, is essential for the bacterium that causes Lyme disease to become virulent. Norgard new_low res Dr. Michael Norgard (right), chairman of microbiology, and Dr. Zhiming Ouyang, a postdoctoral researcher, have identified a protein that may help give Lyme disease its virulent bite.
“We believe our findings provide a foundation for further defining metal homeostasis in this human pathogen and may lead to new strategies for thwarting Lyme disease,” said Dr. Michael Norgard, chairman of microbiology at UT Southwestern and senior author of a study now online and in an upcoming issue of the Proceedings of the National Academy of Sciences.
Lyme disease, discovered in 1977, is the most prevalent tick-borne infection in the U.S. Borrelia burgdorfei, the bacterium that causes Lyme disease, lives in infected mammals and in the midgut of ticks. When an infected tick bites an animal or a human, the bacteria are transmitted to the new host. Infection causes fever, malaise, fatigue, headache, muscle and joint aches, and a characteristic “bull’s-eye” rash that surrounds the site of infection.
To establish infection, however, the bacterium also must acquire a number of essential nutrients, including metals like manganese from its mammalian and tick hosts. Until now, no metal transporter responsible for this acquisition had been identified in this bacterium.
In the current study, microbiologists examined whether bacteria genetically engineered to lack this manganese transporter, called BmtA, transmitted Lyme disease to ticks and mice. The bacterium lacking the transporter, Dr. Norgard said, grows a bit more slowly in the test tube but is not dramatically different from the normal version.
“When you try to grow it in a mouse, however, it can’t grow,” he said. “The fact that the bacterium without this particular manganese transporter can’t grow in a mouse raises important questions about what aspects of physiology and metabolism contribute to the pathogenicity of the organism.”
Lead author Dr. Zhiming Ouyang, postdoctoral researcher in microbiology at UT Southwestern, said another newly discovered characteristic about the bacterium that causes Lyme disease is that it doesn’t seem to require iron to function, something most other pathogens need to survive.
“Out of the thousands of bacteria known, the Lyme disease agent and only one or two other bacterial species do not require iron for growth,” Dr. Ouyang said. “That raises the question as to what other metal co-factors the Lyme disease bacterium depends on to carry out the work that iron does for all these other biological systems. Our research suggests that manganese is a really important one.”
The next step is to understand the exact mechanism of how manganese functions in the organism.
“I really think that there’s also something to the notion that manganese may regulate the expression of other virulence factors,” Dr. Norgard said. “It could be that manganese has more of an indirect effect, but more research is needed to determine what must happen for Borrelia burgdorfei to become virulent.”
U of TX has a pretty good idea...........Come forth my friends, and tell us more.
Seriously! Good for the U of T!!!!! So MANGANESE? I know I have mercury and IRON overload from tests. This proves how you have to get rid of heavy metals to get rid of Lyme and probably Morgellons!
Manganese is in stainless steel, which I use to cook with. Figures, they say it's safer than other metal cookware. Why would I believe that? They are all liars. Dr Mercola sells cookware that has no metals, but very expensive. So that's where I got my metal overload....
The one I found covers the most current line around world, seems to be following shorelines. the b. miyo.......and others, recombined just like influenza........Can see the plan can't we. Update it, constantly, recombine it, give it a different vector. so on.....
Here is what it says: and................I GOT A BIG ONE FOR YA>another matter.....so related, at the core.
"HAMAP Home | Contact HAMAP logo Home Proteomes Families Hamap-Scan Documents Downloads Links HAMAP: Borrelia turicatae (strain 91E135) complete proteome General information
Species: Borrelia turicatae (strain 91E135) Species code: BORT9 Taxonomy: Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Borrelia (TaxID: 314724) [NEWT/ NCBI] Description: Relapsing fever (RF) is a disease caused by several spirochetes of the genus Borrelia. Relapsing fever borrelioses are characterized by recurrent febrile episodes and spirochetemia. There are 2 forms; louse-borne relapsing fever (also known as urban or epidemic RF) is caused by Borrelia recurrentis, and is transmitted by the body louse Pediculus humanus humanus. It currently known in Ethiopia. Endemic tick-borne relapsing fever (TBRF) is a zoonotic disease transmitted worldwide by softbody ticks of the genus Ornithodoros. It is caused by at least 15 distinct Borrelia species throughout the world, including Borrelia hermsii and Borrelia turicatae, carried by the ticks Ornithodoros hermsii and O.turicatae respectively (modified from PubMed 16965346). B.turicatae is pathogenic in dogs and is found in Florida, Texas and Kansas. Strain 91E135 was isolated in 1991 in Texas. Like B.hermsii, B.turicatae evades the mammalian immune system by periodically switching expression among members of two multigene families that encode immunogenic, antigenically distinct outer surface proteins (modified from 16081922). Properties: Presence of flagella: Yes Human pathogen: Probable Interaction: Animal pathogen in Mammalia Number of membranes: 2 Number of inteins:0 Statistics: Number of BORT9 entries in the UniProt Knowledgebase: 818 (115 in UniProtKB/Swiss-Prot + 703 in UniProtKB/TrEMBL)
"....Look familiar? Sounds alot like spirochete + mycoplasma. These Spiroplasma travel in the same spiral drilling motion as spirochetes however hide their protein (identifing markers) from immune system etc by essentially turning inside out...."
Anyone remembers the West Nile Virus...it kills people. We had cases in my area a few years ago, like it came out of nowhere...the symptoms are totally ambiguous...more later (tired). Love.
Anyone remembers the West Nile Virus...it kills people. We had cases in my area a few years ago, like it came out of nowhere...the symptoms are totally ambiguous...more later (tired). Love.
Raf.
what is this about the west nile virus? what connection are you making here?
thanks,
Kritters
Posted by rafael25 on Dec 24, 2011, 1:13pm
Animals are vectors for west nile virus: Mosquito, birds, and even horses.
Mosquito feed from sick animals and then they feed from healthy animals, and the latter become infected.
80% of the people infected don't show any symptoms.
The neurological symptoms can become permanent injuries.
All of the symptoms can easily be misdiagnosed.
STAGNANT WATER rich in organic nutrients is key in the cycle of life of mosquito.
Everything sounds relevant to the conglomerate of creatures and infections that we are observing all around us. I don't know...gimme some time...love. pax. mar.
from your link Kritters::::::::::: just what we want TICK SALIVA>.........
On the Trail of a Vaccine for Lyme Disease: Yale Researchers Target Tick Saliva New Haven, Conn. — A protein found in the saliva of ticks helps protect mice from developing Lyme disease, Yale researchers have discovered. The findings, published in the November 19 issue of Cell Host & Microbe, may spur development of a new vaccine against infection from Lyme disease,...
oh my gosh............the IRON and MANGANESE Connection..............
=========
Scientists identify potential key to Lyme disease
DALLAS — Feb. 9, 2009 — Researchers at UT Southwestern Medical Center have identified a protein that may help give Lyme disease its bite.
The findings suggest that the bacterial protein, which aids in transporting the metal manganese, is essential for the bacterium that causes Lyme disease to become virulent. Norgard new_low res Dr. Michael Norgard (right), chairman of microbiology, and Dr. Zhiming Ouyang, a postdoctoral researcher, have identified a protein that may help give Lyme disease its virulent bite.
“We believe our findings provide a foundation for further defining metal homeostasis in this human pathogen and may lead to new strategies for thwarting Lyme disease,” said Dr. Michael Norgard, chairman of microbiology at UT Southwestern and senior author of a study now online and in an upcoming issue of the Proceedings of the National Academy of Sciences.
Lyme disease, discovered in 1977, is the most prevalent tick-borne infection in the U.S. Borrelia burgdorfei, the bacterium that causes Lyme disease, lives in infected mammals and in the midgut of ticks. When an infected tick bites an animal or a human, the bacteria are transmitted to the new host. Infection causes fever, malaise, fatigue, headache, muscle and joint aches, and a characteristic “bull’s-eye” rash that surrounds the site of infection.
To establish infection, however, the bacterium also must acquire a number of essential nutrients, including metals like manganese from its mammalian and tick hosts. Until now, no metal transporter responsible for this acquisition had been identified in this bacterium.
In the current study, microbiologists examined whether bacteria genetically engineered to lack this manganese transporter, called BmtA, transmitted Lyme disease to ticks and mice. The bacterium lacking the transporter, Dr. Norgard said, grows a bit more slowly in the test tube but is not dramatically different from the normal version.
“When you try to grow it in a mouse, however, it can’t grow,” he said. “The fact that the bacterium without this particular manganese transporter can’t grow in a mouse raises important questions about what aspects of physiology and metabolism contribute to the pathogenicity of the organism.”
Lead author Dr. Zhiming Ouyang, postdoctoral researcher in microbiology at UT Southwestern, said another newly discovered characteristic about the bacterium that causes Lyme disease is that it doesn’t seem to require iron to function, something most other pathogens need to survive.
“Out of the thousands of bacteria known, the Lyme disease agent and only one or two other bacterial species do not require iron for growth,” Dr. Ouyang said. “That raises the question as to what other metal co-factors the Lyme disease bacterium depends on to carry out the work that iron does for all these other biological systems. Our research suggests that manganese is a really important one.”
The next step is to understand the exact mechanism of how manganese functions in the organism.
“I really think that there’s also something to the notion that manganese may regulate the expression of other virulence factors,” Dr. Norgard said. “It could be that manganese has more of an indirect effect, but more research is needed to determine what must happen for Borrelia burgdorfei to become virulent.”
U of TX has a pretty good idea...........Come forth my friends, and tell us more.
Sky, I'm going over all of this and had a few thoughts/questions.
Beginning with:
"...The Yale medical school researchers — Durland Fish, an entomologist, and Dr. Peter J. Krause, an epidemiologist — have recently won a grant from the National Institutes of Health to study the symptoms and develop a rapid diagnostic kit. Dr. Fish found B. miyamotoi in American ticks 10 years ago, but was repeatedly refused a study grant until the Russians proved it caused illness. “It’s been like pulling teeth,” he said. “Go ask the N.I.H. why.” The discovery will no doubt add to the controversy surrounding Lyme disease. While most Lyme victims are cured by a two-week course of antibiotics, some have symptoms that go on for years and believe they have persistent infections that the antibiotics did not reach..."
Yes, let's ask the N.I.H. why!!!
"....On the Trail of a Vaccine for Lyme Disease: Yale Researchers Target Tick Saliva New Haven, Conn. — A protein found in the saliva of ticks helps protect mice from developing Lyme disease, Yale researchers have discovered. The findings, published in the November 19 issue of Cell Host & Microbe, may spur development of a new vaccine against infection from Lyme disease,..."
Did they ever state what that protein is?
Also, I wonder if MERCURY is an implicated metal. With everyone having high levels of mercury, which also has been proven to cause neurological diseases (like Autism) that would be something they would be certain to avoid acknowledging, I'm sure.
It is the coolest!! Check out the people in the photo!
Anyway, look at this: "...Someone will have to design a blood test that will include Borrelia miyamotoi infections. And for doctors who have been relying on a bulls-eye rash for a diagnosis of Borreliosis, it's way past time to look at the bigger picture...."
Is anyone else getting majorly sick to her/his stomach with all of this insideous, evil stuff going on in the world???
So, the protein we are looking for that was used for the vaccine?
protein found in the saliva of ticks helps protect mice from developing Lyme disease,
Looks like they want to use this against myasthenia gravis:
Doctors believe that myasthenia gravis is caused by an overreaction of the complement system, a component of the immune system that specifically defends against parasites, bacteria and other pathogens. Antibodies block nerve receptors at the neuromuscular junction, the place where nerves connect with muscles, and then activate complement which prevents muscle contraction from occurring, causing weakness.
To impede the complement system's misplaced response, researchers hope a new class of drugs, called complement inhibitors, may stop the body's defense system from attacking itself. Other researchers discovered that rEV576, a protein found in tick saliva, works as a complement inhibitor, allowing ticks to avoiding setting off an immune response in their human host.
Amblyomma cajennense tickHow Does it Work?According to AFP, the Factor X active protein in the tick spit "shares some characteristics with a common anti-coagulant called TFPI (Tissue Factor Pathway Inhibitor), specifically a Kunitz-type inhibitor which also has been shown to interfere with cell growth." Since a cancer is basically a group of cells that are growing out of control, controlling this growth is very important.
I think we are talking wood ticks............the wood inside the tick, if they live on trees, or wood, life cycle of wood ticks, what else do they collect in their saliva? They must have the spirochete itself. the saliva delivers it to us. Is this nuts, or what? Why would you use the source of the saliva vector itself, which carries the spirochete?
[i]lignin does not break down, ...... And this tick shown above looks like a cross between reduviid bug and tick. I did not think ticks had that raised membrane around the body.
this is interesting.
It does have a signature: Protein bank?
rEV576, a protein [/i]
They want to use it for Myasthenia gravis and Guillian Barre......both of these resemble Lymes, don't they?
"Munich/Jena, Germany, October 12, 2006 - An agreement signed between Wacker Biotech GmbH and Evolutec Group plc (AIM code: EVC) contracts Wacker Biotech as partner for the process development and GMP-compliant production of Evolutec's protein drug development candidate rEV576 for clinical trials. The manufacturing process will use WACKER's E. coli secretion technology which - in a feasibility study - was shown to be an enabling technology for production of rEV576.
Under the terms of the agreement, Wacker Biotech will conduct full analytical and process development services leading to a robust and scaleable process to manufacture rEV576 drug substance according to current GMP (Good Manufacturing Practice) for Phase I clinical trials which are expected to commence in 2007. rEV576 has demonstrated efficacy at the preclinical stage in several indications, including the severe autoimmune diseases myasthenia gravis and Guillain-Barré Syndrome.
It looks like they use the e-coli to grow this product. But, the e-coli is only the product container. So, this spit product can be grown in e-coli and sold as secretion agent.
.The core of the technology is WACKER's proprietary E. coli K12 strain, which is capable of secreting recombinant proteins in their native form into the fermentation broth during cultivation. Extracellular production facilitates straightforward purification of recombinant products, eliminates tedious refolding steps and makes the whole manufacturing process more efficient and cost effective.?
Wacker Biotech has already conducted a feasibility study with rEV576 which demonstrated that WACKER's secretion technology produces promising yields of active protein. Dr. Thomas Maier, Managing Director of Wacker Biotech, commented: "There was already evidence from other projects that our secretion technology is a very cost-efficient means of producing therapeutic proteins. We are very pleased that it proved to add value to the development of rEV576."
Mark Carnegie Brown, Evolutec's Chief Executive Officer, commented: "Promising preclinical data and progress made by Wacker Biotech points to the commercial potential of rEV576. We look forward to maintaining this momentum in the development programme."
About Evolutec Evolutec, which is based in Reading, UK, is a clinical stage biopharmaceutical company with a focus on allergy, inflammation and autoimmune diseases. Evolutec is listed on the AIM market of the London Stock Exchange and develops therapeutics originally isolated from the saliva of ticks.
Wacker Biotech has already conducted a feasibility study with rEV576 which demonstrated that WACKER's secretion technology produces promising yields of active protein. Dr. Thomas Maier, Managing Director of Wacker Biotech, commented: "There was already evidence from other projects that our secretion technology is a very cost-efficient means of producing therapeutic proteins. We are very pleased that it proved to add value to the development of rEV576."
Mark Carnegie Brown, Evolutec's Chief Executive Officer, commented: "Promising preclinical data and progress made by Wacker Biotech points to the commercial potential of rEV576. We look forward to maintaining this momentum in the development programme."
About Evolutec Evolutec, which is based in Reading, UK, is a clinical stage biopharmaceutical company with a focus on allergy, inflammation and autoimmune diseases. Evolutec is listed on the AIM market of the London Stock Exchange and develops therapeutics originally isolated from the saliva of ticks.
About Wacker Biotech Wacker Biotech GmbH is an experienced full-service contract manufacturer of biopharmaceuticals derived from microbial systems. Its portfolio ranges from services in molecular biology, analytical and process development, through GMP-compliant production of clinical test material and drug substance for commercial market supply. Above all, Wacker Biotech offers proprietary technologies that satisfy market needs for cost-efficient and high-quality production. Two examples are its E. coli-based secretion system and the high-cell-density fermentation technology. Jena-based Wacker Biotech is a wholly-owned WACKER subsidiary, operating since early 2005 as the direct successor of ProThera GmbH.
More New Product News from this company: UV-Active Silicones target microelectronics industry. Silicone Rubber is suited for medical device manufacturing. Process-Stable Hydrophilic Softener suits textile finishing. More .... Other News from this company: WACKER Intensifies Collaboration with Russian Silicone Producer WACKER Receives Innovation Award for Alpha-Silane-Based Adhesives and Sealants Wacker Chemical appoints BSI exclusive West Coast Distributor Wacker Acquires Silicon-Metal Production Site in Norway WACKER Appoints New Distributor for Polymer and Silicone Products in Chile
Looks like the Bank of London is making money of Tick spit. How ingenious.
UV-Active Silicones target microelectronics industry. rubber....... Alpha-Silane-Based Adhesives and Sealants....... silicon................. gee what do they have to do with tick spit?
![[Home]](http://images.proboards.com/menu/home.gif "[Home]")
![[New Topics]](http://images.proboards.com/menu/topics.png "[New Topics]")
![[Help]](http://images.proboards.com/menu/help.gif "[Help]")
![[Search]](http://images.proboards.com/menu/search.gif "[Search]")
![[Login]](http://images.proboards.com/menu/login.gif "[Login]")
![[Register]](http://images.proboards.com/menu/register.gif "[Register]")
![[Live Chat]](http://images.proboards.com/menu/chat.gif "[Live Chat]")
